LOCUS CK335223 521 bp mRNA linear EST 12-MAY-2010 DEFINITION H3153G04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3153G04 3', mRNA sequence. ACCESSION CK335223 VERSION CK335223.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 521) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3153 row: G column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..521 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3153G04-3" /db_xref="taxon:10090" /clone="H3153G04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 148 a 125 c 128 g 120 t ORIGIN 1 ggggaggaaa gggtttattc agcttacact tccacattgc tgttcatcac taaaggaagt 61 cgagaatgga actcaagcag gtcaggaagc aggagctggt gcagaggcta tggagggatg 121 ttctttactg gcttgcttcc cctggcttac tcagcttgct ctcttataga accaagacta 181 ccagcccaga gatggtccca cccacaaggg acctttcccc cttgatcact aattaagaaa 241 atgccttaca gctggatctc atggaggcat ttcctcaact gaagctcctt tctctgtggt 301 aactccagct gtgtcaagtt gacacaaaac tagccagtac agtacctatt tctcaaggaa 361 ctcacagtac cttttgctca cagaaggggt taggtttagg caggaatgtc tgtggccaca 421 atgattacag gcagcaagaa agcagaagca aggagaactg acaccaagac acggcacctg 481 ggtcactaag gatgagccct ttaaaatgca ggagggacag c //