LOCUS CK335074 639 bp mRNA linear EST 12-MAY-2010
DEFINITION H3139D01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3139D01 5', mRNA sequence.
ACCESSION CK335074
VERSION CK335074.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 639)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT Contact: Dawood B. Dudekula
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
Plate: H3139 row: D column: 01
Seq primer: M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..639
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3139D01-5"
/db_xref="taxon:10090"
/clone="H3139D01"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 169 a 160 c 145 g 165 t
ORIGIN
1 gtgaagcaaa ggctatttat ccctttgcct tctcaccatc acggtttgcc aacagcatgg
61 ccctgcgcat gcgtgaacct aatccacaaa tgtgcaagtg gcaggtgaca caggagaaac
121 aatggagcca gatttcctgg ggggagaagc agtctcctga gctctctttt cctagctgga
181 aaccaaacgt tcattcagga tcactccctt accctgccca gttcccaaca attccagaag
241 aagagggaag gaaagtgtgt ctccagggac ttgctctttg gctattttct gacacagtgg
301 acttctaaag gatgtcaaga aggctgcttc ctagggtggc tatacttttc ctgtccagag
361 gcttcaaagc acactgtcaa acttctggta aggccactcc acccttcaca ccctttcctg
421 cgagggatta gacagcagat ttccttaact ttcctcccag cccctaccca aaagcaaatg
481 cgttttggat ttgtaacttt ccttctatca acagaattgc atggcaagtt acgtatttgg
541 tctcagtatt tccaaagaga aaggaaagta cttgttccat ggctgagggt gtacaaagaa
601 tagaagtcag gggatgagtg gggatccctc cacaagatt
//