LOCUS CK335048 590 bp mRNA linear EST 12-MAY-2010
DEFINITION H3135H02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3135H02 5', mRNA sequence.
ACCESSION CK335048
VERSION CK335048.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 590)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT Contact: Dawood B. Dudekula
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
Plate: H3135 row: H column: 02
Seq primer: M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..590
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3135H02-5"
/db_xref="taxon:10090"
/clone="H3135H02"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 184 a 129 c 153 g 124 t
ORIGIN
1 ccatcataag ttccttcatt cacgaaagct ccatgtcccg ggcacagtct cctccagtgc
61 ctgcaaggaa gaaccaactc cgtgcagaag aggagaaaaa aaatgtgatt atggagttat
121 cagaaatgag gaagcagctc cggagcgagg agaggcgttt gcaggggcgg ctgctgcacc
181 tggacagtga cgacgagatc cccatgagga aaagagagag gaacccaatg gatatatttg
241 acatggctag acatcgggta caagcccctg tcagaagacc atcgcctaag ggcttagatg
301 ctactacttt tcagaatatt catgacttta atgagctgag agagagagat tcagatactc
361 gagtggatct gagattaatg tacccagatc ctccccgcga tcatcacacc ttggagattc
421 agcagcaggc cctactccga gagcagcaga agaggcttaa tagaataaag atgcggagag
481 atgcaggagc tgatttgggc accatctgca ctgataatgc acaagggcgc agaatgccca
541 gagatgacac taatgatttt ttgaaaaatt cattactaga atctgatagt
//