LOCUS CK335035 371 bp mRNA linear EST 12-MAY-2010 DEFINITION H3133G04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3133G04 5', mRNA sequence. ACCESSION CK335035 VERSION CK335035.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 371) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3133 row: G column: 04 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..371 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3133G04-5" /db_xref="taxon:10090" /clone="H3133G04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 80 a 100 c 102 g 89 t ORIGIN 1 gctgcggccg ggtcggcttt ttgcgctgta gtggtctctg cggttccttg gaagcacagc 61 tccccttccc cgccccagtc ccagtccccg tccggccgcg gacataaaga tgagtagctc 121 tgaggaggtg tcctggattt cctggttctg tgggctccgt ggtaatgaat tcttctgtga 181 ggtggatgaa gactacatcc aggacaaatt taatcttact ggactcaatg agcaggtgcc 241 tcactatcga caagctctgg acatgatctt agacctggaa cctgatgaag agctggaaga 301 caaccccaac cagagcgact tgatcgaaca ggcagctgag atgctttatg ggttgatcca 361 cgcccgctac a //