LOCUS CK335002 571 bp mRNA linear EST 12-MAY-2010 DEFINITION H3131E07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3131E07 5', mRNA sequence. ACCESSION CK335002 VERSION CK335002.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 571) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3131 row: E column: 07 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..571 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3131E07-5" /db_xref="taxon:10090" /clone="H3131E07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 136 a 181 c 154 g 100 t ORIGIN 1 ctttagcgcg ccgtgggctc agctgcgacc ccggccccgc ccccgggacc ctgaccatgg 61 acgatctgtt tcccctcatc tttccctcag agccagccca ggcttctggg ccttatgtgg 121 agatcatcga acagccgaag caacggggca tgcgattccg ctataaatgc gaggggcgct 181 cagcgggcag tattcctggc gagagaagca cagataccac caagacacac cccaccatca 241 agatcaatgg ctacacagga ccaggaacag ttcgaatctc cctggtcacc aaggatccac 301 ctcaccggcc tcatccacat gaacttgtgg ggaaggactg ccgggatggc tactatgagg 361 ctgacctctg cccagaccgc agtatccata gcttccagaa cctggggatc cagtgtgtga 421 agaagcgaga cctggagcaa gccattagcc agcgaatcca gaccaacaat aacccctttc 481 acgttcctat agaggagcag cgcggggact atgacttgaa tgcagtgcgc ctctgcttcc 541 aggtgacagt gcgggaccca gcaggcaggc c //