LOCUS CK334860 590 bp mRNA linear EST 12-MAY-2010
DEFINITION H3117G03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3117G03 5', mRNA sequence.
ACCESSION CK334860
VERSION CK334860.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 590)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT Contact: Dawood B. Dudekula
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
Plate: H3117 row: G column: 03
Seq primer: M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..590
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3117G03-5"
/db_xref="taxon:10090"
/clone="H3117G03"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 128 a 129 c 168 g 165 t
ORIGIN
1 ctgtctgcca gcagaggaat ctcctcccct gggcacagcg gctcctttct ctctgtgctg
61 ccaagtgcaa gtctgagaag cacagcacta aaagaggcta tgcagaggca gagagcgtgt
121 gagaaagctc acactttggc agaggtgctg ggaagtagtg tggcgagggc aggcacaaga
181 ggcatcctat gtgtgtcttt gtggtccagg cgtcccccct ctctctgcca gcttgaggtg
241 taggcttgat aactaaatta gagacctccc ccctttgatt gacaacttag taattagaga
301 gatactaatc tgctgtcttg ttgcgtcttg gttttagcag aaaaataagc ggaggaggag
361 gcctttattg ttgctgtttc ttttgtggtg tggccagaga tatctggaac ctcagggtag
421 aagtgggggc tcagccctga atggtgattt cccaccgtgc taagcttgtc ttgccacctt
481 tgctttttca tctttgcgtc atagaggagg ctgagtggcg tttgccccat tttttttttt
541 gcagaatcag gaaggtaaag tggtctgctc caggccagat agtggtgaaa
//