LOCUS CK334824 600 bp mRNA linear EST 12-MAY-2010 DEFINITION H3114A01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3114A01 3', mRNA sequence. ACCESSION CK334824 VERSION CK334824.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 600) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3114 row: A column: 01 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..600 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3114A01-3" /db_xref="taxon:10090" /clone="H3114A01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 124 a 172 c 159 g 145 t ORIGIN 1 cacatttcta cattttattc ccacaaggca gccagggtga agctcgagcc cagggccaca 61 ggcctctgga ccttcctccg gcagggatgc acgccaggtc ctcaggactg ccccaccagc 121 aggctggttt tcaggcagac cttcatgagt gccggggtgg aaggctccag gggtcacagg 181 gcaggggggc tcggtgtagg ggcacacact tgtagggcta gctagagata gcacggcagg 241 tccttctcta tcacctgggg ctcctccatg ggaccatagc gcttcaccac gcagccgttc 301 ttatcaatga gaaacttggt aaagttccat ttgatggcat ttcccagcat gcccctgccc 361 ttgggctgga ctttcatcca tttccacagt gggtgggcat cgtccccatt tacacagatc 421 ttgctgtaca cgtcaaactt gacgttgtag ccggctgcaa actccttgat ttcttgatta 481 cttcctggct cctgcctccc aaactggttg caggggaagg ccaggattag taaaccacac 541 tcagcatatc gggcatgcag atcgactagc tgagtgtagt ttacgtcagt tttgcctcat //