LOCUS CK334726 559 bp mRNA linear EST 12-MAY-2010 DEFINITION H3100D04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3100D04 5', mRNA sequence. ACCESSION CK334726 VERSION CK334726.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 559) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3100 row: D column: 04 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..559 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3100D04-5" /db_xref="taxon:10090" /clone="H3100D04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 152 a 127 c 143 g 137 t ORIGIN 1 ctcgagtcgg ggctggtgac agtcaactga aaggcgtctc cacattcatg gctgaaatgc 61 tggagactgc ttccatcctc aggtcagcaa ccaaagactc cttaataatc attgatgagc 121 tgggaagagg aacctctacc tatgatggat ttgggttagc atgggctata tcagattaca 181 ttgcaacgaa gattggtgcc ttttgcatgt ttgccaccca ttttcatgaa cttactgctt 241 tggccaacca aataccaact gttaataatc tacatgtcac agcgctcact actgaggaga 301 ccctaactat gctttaccaa gtgaaaaaag gtgtctgtga tcagagtttc gggattcacg 361 tggctgagct cgctaacttc ccgaggcacg tgatagcgtg cgccaagcag aaggctctag 421 agcttgaaga atttcagaac attggaacct cgctgggatg tgacgaagcc gagccggctg 481 caaagagacg ctgcctggaa agagagcaag gtgagaaaat tattctggag ttcctgtcga 541 aggtcaagca ggtgccctt //