LOCUS CK334724 461 bp mRNA linear EST 12-MAY-2010 DEFINITION H3100B01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3100B01 5', mRNA sequence. ACCESSION CK334724 VERSION CK334724.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 461) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3100 row: B column: 01 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..461 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3100B01-5" /db_xref="taxon:10090" /clone="H3100B01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 107 a 155 c 103 g 96 t ORIGIN 1 ggagcatccc agcctcctgt ccacaggcct gtgaggtctc tcaggcccag ttgcacagca 61 tccaagcctc ttctaggctg ggagcctctc cacttctccc tgtggtcctc ccatcatcac 121 tcaagcagcc acagtccacc tccaaagaaa agaagtaact gcctttcctt gtccctggtc 181 atgacttaaa tcacagcaga gaaaaggaaa gcacttcagg agggccagca ccacagtggg 241 cagcagcagt gtcacttcta ccagcagcct aggtgactgt cagtggacat gcaggccatg 301 tggacagcct gttacctcaa aacacccaga acaagcacgc cttcccagga ggccacttgt 361 cccctactct gtcagagcct tggcttctca actccattct gctcgctgct ggggctcctc 421 tctatcagct tggttcacag cacagcccac ctgaagagca g //