LOCUS CK334645 598 bp mRNA linear EST 12-MAY-2010
DEFINITION H3074A05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3074A05 3', mRNA sequence.
ACCESSION CK334645
VERSION CK334645.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 598)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT Contact: Dawood B. Dudekula
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
Plate: H3074 row: A column: 05
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..598
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3074A05-3"
/db_xref="taxon:10090"
/clone="H3074A05"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 189 a 122 c 134 g 153 t
ORIGIN
1 agtatttaaa aggtaactat acacatttta ttttcataca tttaaaaaca acatatatgt
61 gtgtgaagag cagactgaca ggttttcaag atcagtcagt gtgggcagaa ctggcacaac
121 agacagaata aatatagtgc tttggggaag agtagtgatg ggtttggaga gcctcattgt
181 ggacgtgatt acacagataa cttgtagatg tgcaaagttg ctgactccat gacaggaatg
241 gcccagccca ccagacagcc tctctatgtg gttgtcatct acagggaaca gactgggcct
301 aatgagcaca gggttgggag actggagaaa aactcaacag gtcagacaag catgtgtgtt
361 cacacatata cctcaagaaa ttgcactaca gaaatgactg ctactctttt tggcagcagg
421 tcaggccatc tcatactgga actcaagaaa ccctcactgg gatagtgcca gccaggtaca
481 cgcctctggg tgacatcaaa gcttccatag ctacagcatt aatcaaatgt ccgcgaacaa
541 aaatatatat atttttagat gttaccagaa ttacttcatg tcttgtaccc ctgtatgc
//