LOCUS CK334543 742 bp mRNA linear EST 12-MAY-2010
DEFINITION H3049A04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3049A04 3', mRNA sequence.
ACCESSION CK334543
VERSION CK334543.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 742)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT Contact: Dawood B. Dudekula
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
Plate: H3049 row: A column: 04
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..742
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3049A04-3"
/db_xref="taxon:10090"
/clone="H3049A04"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 156 a 216 c 215 g 155 t
ORIGIN
1 ctccagcacc tggaactgtt tattggacat tgttgccagt tgcaggacag gaagctagac
61 agaagaaaaa cagtgaccat agacccatag ggacagacct tgcctagatg gggacatttg
121 tatccccaac ccggtggacc aacctggcaa ctgctgcccc tccccaggcc cccaggactt
181 tggcataacg ctgatggggg taggaggctg cagacagcag agcccctcaa cccgaagcag
241 agagtctgat gagcgctggg gacaggtggg aggagagggg acacggggca ggccatgggc
301 caggatgtga ggagcagcac aagtgaagaa gttgggagct agggacatgg tggctaagtt
361 tccttttccg gagaagggtc acgcactgac gtctttctcg ttgcccgctt tggggacggc
421 ttccagcaat gggtccccgg ggcccgcctc ctccccctcc ttggcctcac cagacttgga
481 gttggggacc cagaggccag aatcgatgca acgctgcatg tgatacttgg catcagtggg
541 atccattttg ctgatggcgt cttgcagcat ctgtacatcc ttcacatcaa agcacttctg
601 cagctcctca ggcagggatt cgtagacctc cacggggtcc aggccaccag ggcctagcct
661 cttcttgcgc tcctcctctt catattcctt catggccttc tctattcgca gcttggcgcg
721 gccccgcact cgctccttaa ag
//