LOCUS CK334375 661 bp mRNA linear EST 12-MAY-2010
DEFINITION H3020D07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3020D07 5', mRNA sequence.
ACCESSION CK334375
VERSION CK334375.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 661)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT Contact: Dawood B. Dudekula
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
Plate: H3020 row: D column: 07
Seq primer: M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..661
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3020D07-5"
/db_xref="taxon:10090"
/clone="H3020D07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 202 a 151 c 164 g 144 t
ORIGIN
1 aacgcaggtg ttgtgaaaac caccgctaat tcaaagcaaa aatgggaaag gaaaagactc
61 acatcaacat cgtcgtaatc ggacacgtag attccggcaa gtccaccaca accggccacc
121 tgatctacaa atgtggtgga atcgacaagc gaaccatcga aaagtttgag aaggaggctg
181 ctgagatggg aaagggctcc ttcaagtacg cctgggtctt agacaaactg aaagctgagc
241 gtgagcgtgg tatcactatt gacatctccc tgtggaaatt cgagaccagc aaatactatg
301 tgaccatcat tgatgcccca ggacacagag acttcatcaa aaacatgatt acaggcacat
361 cccaggctga ctgtgctgtc ctgattgttg ctgctggtgt tggtgaattt gaagctggta
421 tctccaagaa cgggcagacc cgcgagcatg ctcttctggc ttacaccctg ggtgtgaaac
481 agctgattgt tggtgtcaac aaaatggatt ccaccgagcc accatacagt cagaagagat
541 acgaggaaat cgttaaggaa gtcagcacct acattaagaa aattggctac aaccctgaca
601 cagtagcatt tgtgccaatt tctggttgga atggtgacaa catgctggag ccaagtgcta
661 a
//