LOCUS CK334353 693 bp mRNA linear EST 12-MAY-2010 DEFINITION H3019A07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3019A07 5', mRNA sequence. ACCESSION CK334353 VERSION CK334353.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 693) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3019 row: A column: 07 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..693 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3019A07-5" /db_xref="taxon:10090" /clone="H3019A07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 222 a 134 c 173 g 164 t ORIGIN 1 tgttctcaga agtcctggag aaccagttcc tccaggccgc taagctggtg gagaatcact 61 tggactctga aattcagaaa ctggatcaga ttggtgaaga tgaactggag ctgctcaaag 121 aaaagaggct ggcggcgctc aggaaggttc agcagcagaa acaagagtgg ctttccaaag 181 ggcacgggga gtacagagaa atcggcagcg agagagactt ctttcaagag gtcaaggaga 241 gtgagaaagt ggtttgccat ttctacagag acaccacatt caggtgtaaa atactagaca 301 gacatttggc aattctggcc aaaaagcatc ttgaaaccaa gtttttgaag ctgaatgtgg 361 aaaaggcacc attcctctgt gagagactgc ggatcaaggt catccccacg ctagctctct 421 taagagatgg caagacgcaa gattatgttg ttggattcac cgacctagga aatacagatg 481 acttcactac agaaacttta gaatggagac ttggttgttc tgatgttatc aattacagtg 541 gaaatttaat ggagccacca tttcagagcc aaaagaaatt tgggacaaac tttacaaagc 601 tggaaaagaa aactatccga ggaaagaaat acgattccga ttctgatgat gactagactg 661 tgctgtactt taaaaccttc cgtctgtctg tct //