LOCUS CK334314 582 bp mRNA linear EST 12-MAY-2010 DEFINITION H3016B09-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3016B09 5', mRNA sequence. ACCESSION CK334314 VERSION CK334314.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 582) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3016 row: B column: 09 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..582 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3016B09-5" /db_xref="taxon:10090" /clone="H3016B09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 133 a 190 c 139 g 120 t ORIGIN 1 cattgaccag agcactctgg agaagtgtaa cctcagtgaa gctgcttggg gccgagtgcc 61 cagtgaccct ttcacagggc tagcctttac tccacagtcc cagcccctcc ctcatccttc 121 cctgaaagcc cggatcgacc ggttcctact ccagcactct atttcaggct gccgcctgct 181 tgggagagca cagactccat cagtaatgac cccttctgtc attaccctgc cctcaaggaa 241 aaggaagaca gagcaggctg aacacagctc acactacagc cttggcatga gtgcgtcttc 301 ctctgccaca agccctttat tctcacctac tacctcagag ccaactgcca agaagatgaa 361 agccaccagt gagcttggcc tgacggacat ggactgttca gcaggtccag tgtcccatga 421 gcagaagctg gcgcaaagtc tggaaattgc cctgacttct acccttggct ccatgccttc 481 cttcactgcc cggctcacca aagggcagct ccagctgggc acaagaggga gcagtgcttg 541 caggaggcca gcctccagct ccgagcatcc aaggagtgtt tc //