LOCUS       CK334278                 593 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3012F08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3012F08 5', mRNA sequence.
ACCESSION   CK334278
VERSION     CK334278.1
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     Contact: Dawood B. Dudekula
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            Plate: H3012  row: F  column: 08
            Seq primer: M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3012F08-5"
                     /db_xref="taxon:10090"
                     /clone="H3012F08"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          150 a          140 c          161 g          142 t
ORIGIN      
        1 cgactagggt cggctctggg cctgacccag gaagtcgggg tcttcttctt ctgtcttttt
       61 ccgtggtact ggcaggattg tgtgggggaa actcagtgga gaggaaaatc tacattccct
      121 taaataaaac agctccttgt gtccgcctgc tcaacgccac tcatcagatt ggctgccagt
      181 agataaaaga tctgcagaag ctggatgtgg tggccttgaa tcgcaggact tggaaagcag
      241 aggcaggagg atcactgcaa attcaaggcc agcttggtct acacagtcag ttcagcttca
      301 attagtgggg atacaggggt tatccatgta gtggagaaag aagaagacct gaagtgggtg
      361 ttgaccgatg gccccaaccc cccttacatg gttctgctgg agggcaagct cttcaccaga
      421 gatgtaatgg agaagctgaa gggaacaacc agtagaatcg ctggtcttgc cgtgactcta
      481 gccaagccca actcaacttc aagcttctct cctagtgtgc agtgcccaaa tgatgggttt
      541 ggtatttact ccaactccta cgggccagag tttgctcact gcaagaaaac act
//