LOCUS CK334270 611 bp mRNA linear EST 12-MAY-2010 DEFINITION H3012E08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3012E08 5', mRNA sequence. ACCESSION CK334270 VERSION CK334270.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 611) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3012 row: E column: 08 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..611 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3012E08-5" /db_xref="taxon:10090" /clone="H3012E08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 156 a 134 c 168 g 153 t ORIGIN 1 ggaaattacg tcattcagaa gttttttgaa tttggcagtc atgaacagaa gctggctctg 61 gctgaacgga ttcgaggcca tgtcctatca ttggcactgc agatgtacgg ctgccgagtc 121 atccagaaag ctttggagtt tattccctca gaccagcaga acgagatggt gcgggagcta 181 gatggccatg tcctgaagtg tgtgaaagac cagaacggca accatgtggt tcagaagtgc 241 attgagtgtg tacagcccca gtctttgcag tttatcatcg atgcattcaa ggggcaggtg 301 tttgctttgt ctacgcaccc gtacggctgt agggtgatcc agaggatcct agagcactgt 361 ctccctgacc agaccctccc catcctggag gagcttcacc agcacacaga gcagcttgtg 421 caggatcagt atggaaatta tgtaatccaa catgtactgg agcatggtcg tcctgaggat 481 aaaagcaaaa ttgtagcaga gatccgaggc aatgtacttg tattgagtca gcacaagttt 541 gcaagcaatg ttgtggagaa gtgtgttact cacgcctcac gtacagagcg tgctgtgctc 601 attgatgagg t //