LOCUS CK334190 660 bp mRNA linear EST 12-MAY-2010
DEFINITION H3007H09-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3007H09 5', mRNA sequence.
ACCESSION CK334190
VERSION CK334190.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 660)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT Contact: Dawood B. Dudekula
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
Plate: H3007 row: H column: 09
Seq primer: M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..660
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3007H09-5"
/db_xref="taxon:10090"
/clone="H3007H09"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 233 a 128 c 173 g 126 t
ORIGIN
1 gagaaaacca gcaatggctt gtgtatgatc agcagcgaga ggcctacgtt aaagggcttt
61 tagcaaagat cttcgagctg gaaaagagaa cagagacagc cgctgcttcc ctcacacagc
121 agatgaagaa gattgaatca gaaggttatc ttcaagtgga gaagcaaaaa tatgatcatc
181 tcttggaaaa tgccaaaaaa gatctcgaag ttgagcgaca agccgtgact cagttgcgtt
241 tagaactcga tgaatttcga aggaaatacg aagaagctcg aaaagaagtt gaagacttaa
301 atcagctgtt gagctctcag cgaaaggcag acatccagca cctggaggag gacaagcaga
361 agacggagag gatccaaaag ctgagggaag agagcagtat cttcaaggga aagctggaag
421 aggagaggaa gaggtcggag gagctcttat cccaggtccg aattctctac gattctctgt
481 taaagcacca agaggagcag gccagggtag ctctactgga acagcagatg caggcatgta
541 ctctagactt tgaaaatgaa aaactcgacc gccagaatat gcagcatcaa ctctatgtga
601 ttcttaagga actccgaaaa gcaaaaagtc aaataacaca gttggaatcc ttgaagcagc
//