LOCUS CK334185 617 bp mRNA linear EST 12-MAY-2010 DEFINITION H3007H02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3007H02 5', mRNA sequence. ACCESSION CK334185 VERSION CK334185.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 617) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3007 row: H column: 02 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..617 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3007H02-5" /db_xref="taxon:10090" /clone="H3007H02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 125 a 180 c 165 g 147 t ORIGIN 1 ggactggaag aagctccaag acctattgag tggcgtgaac cagtactcca cggcatttgg 61 gcgcatctgg ctgtcagtag tgttcgtctt ccgggtgctg gtgtatgtgg tggctgccga 121 gcgtgtgtgg ggtgacgagc aaaaagactt tgactgtaac accaggcaac ccggctgtac 181 caacgtgtgc tatgacaact tcttccccat ctccaacatc cgactctggg ccctgcagct 241 catcttcgtc acgtgtccgt ctatgctggt catcctgcat gtagcctacc gcgaggagcg 301 ggaacggaag catcgccaga agcacgggga gcaatgcgcc aaactgtaca gccacccggg 361 caagaagcat ggcggcctgt ggtggaccta cttgtttagc ctcatcttca agctcatcat 421 cgaattggtc ttcctgtacg ttctccacac gctctggcat ggcttcacca tgccgcgtct 481 ggtacagtgc gccagcatag taccctgccc caacaccgtg gattgctaca tcgctcggcc 541 cacggagaag aaggtcttta cctacttcat ggtaggcgct tctgccgtct gcattattct 601 caccatctgt gagatct //