LOCUS BG088797 590 bp mRNA linear EST 12-MAY-2010
DEFINITION H3157F10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3157F10 5', mRNA sequence.
ACCESSION BG088797
VERSION BG088797.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 590)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG088797.1.
Other_ESTs: H3157F10-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3157 row: F column: 10
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..590
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3157F10-5"
/db_xref="taxon:10090"
/clone="H3157F10"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 159 a 95 c 129 g 207 t
ORIGIN
1 attctgacga gatcatggtc tttttggcag gtaacctaaa tatttgatta aagtattctt
61 taggtgtgtt gcttctgaac agctatttgt atctggttat tttgtgtggc aaagccatct
121 tttatctttg catgtctatg aggattttac agaaatcatt cctgtttacc cctttctctg
181 aaacaaacta atctttttca ttcataatgg actaatttgc aagatgtgag aggaggtggt
241 ttagcatgag tgacaaacat gcatttgtgt tttcatgaaa aaaccttggg cttctaagta
301 ggttaaatgc ttagccggac tgaaataaat agaaggtgag tgtctaattg tatcatgttg
361 gactgtaaat ctttatcact gcttgcttta tggctgggtc agttgacagt ttgagactgt
421 taaatctcat acagaatttc acagttgcga ggttatatgt cagattgagg cgcgcgctac
481 aagatttagg tcagaagccc ttatctgaga gtgggttcca agcctgctta cataaagcag
541 tgttatcagc ctctttatta tttatgtgca tggggcaata taggacacca
//