LOCUS BG088679 689 bp mRNA linear EST 12-MAY-2010 DEFINITION H3156C04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3156C04 5', mRNA sequence. ACCESSION BG088679 VERSION BG088679.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 689) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG088679.1. Other_ESTs: H3156C04-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3156 row: C column: 04 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..689 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3156C04-5" /db_xref="taxon:10090" /clone="H3156C04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 249 a 96 c 121 g 223 t ORIGIN 1 aacattgtgg tttttttcta cattattaat ggaaagaaaa tatcctttaa acaatgtatg 61 taatatgtaa tgtactgttg aaatgggcat tacaacttta tataaccatt ttagggtaaa 121 tatattttat aagtacctat ttaatcttac ttttgtagtt aaatgtactt tttaaaggtt 181 caatctgaaa gtctgttatc atagaaaaat aaattgtatg ttgactcagt tgtatactga 241 atacattttc cctttcctaa gcagacgttt gatagaggca gttgaaacta taagcaagct 301 aagactacta cacattctta tttcctttct atttatgctt tatcttattt taaaaagaaa 361 aacaaaaatt ttctaaacat gtcattgaag gaaattgttt ttttctgcga tagttaagaa 421 gtgacagttg gtcaaaatat agttgaaaac aaacaaaaac ttggtttctg caggatgtgg 481 tagcacacac agtgctcagg aagctaaaac aagaggctca atggtttgaa gccagccaaa 541 actacatagc aaggtcctat ctttaaagat aagagaaaaa tagaggtggt ggaggagaga 601 tcagacaaca ccaagaataa gaaatcgatt cttagccata tttaatggac aaacctgtca 661 tctcagcttt tgggagatag aggcagaag //