LOCUS BG088545 684 bp mRNA linear EST 12-MAY-2010
DEFINITION H3154E01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3154E01 5', mRNA sequence.
ACCESSION BG088545
VERSION BG088545.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 684)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG088545.1.
Other_ESTs: H3154E01-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3154 row: E column: 01
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..684
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3154E01-5"
/db_xref="taxon:10090"
/clone="H3154E01"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 245 a 123 c 188 g 128 t
ORIGIN
1 agaaagcgtg tgagatccga gatcagatta cgagtaagga agcccagtta gcgtcttcgc
61 aggaaattgt caggtcctac gaggatgagc ttgagccatt gaagaatcgc ctgaaagaga
121 ttgaacacaa cctgtctaaa ataatgaaac ttgataatga aattaaagcc ttggagagcc
181 gaaagaaaca aatggagaaa gataacagtg aattagaaca gaaaatggaa aaggtttttc
241 aagggactga tgagcagcta aatgacttgt atcacaatca ccagagaaca gtaagggaga
301 aagaaaggcg cctggtagac tgccagcgag aactggagaa gctgaataaa gaagcacggc
361 tcctcaacca ggaaaaagca gagctgcttg tggagcaggg tcgtctacag ctacaggcag
421 atcgccatca agagcacatc cgagccagag actcattaat tcagtctttg gcaacacatc
481 ttgaattgga tggttttgag cgtggaccat tcagtgaaag acagattaaa aattttcatg
541 aacttgtgaa agagagacag gaaagagaag ctaaaactgc cagccagctc ttgagcgacc
601 tgacagacaa agaagctctg aagcagaggc agcttgatga gctgagggac aggaagagcg
661 gactggggag gacgattgag ctga
//