LOCUS BG088521 708 bp mRNA linear EST 12-MAY-2010 DEFINITION H3154B05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3154B05 5', mRNA sequence. ACCESSION BG088521 VERSION BG088521.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 708) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG088521.1. Other_ESTs: H3154B05-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3154 row: B column: 05 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..708 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3154B05-5" /db_xref="taxon:10090" /clone="H3154B05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 132 a 203 c 173 g 200 t ORIGIN 1 agtccctgaa gacacagggt ctttggctgg tttctgtttg gattctgttt ctgtccccct 61 tttaacaatg tgaagtgact gaggttggat ttcctcttcc tgctacccca agcccccaca 121 tctctttaac tgccagtcca gtgtccacca gccatttgtt ctgacatcct ggccatgcaa 181 gggtcccaag acgggcacaa agtgggtcca ggcatgggag gtggccttgg tcaatgtcac 241 agagctgttg gaagcagtgc caagagctaa attcaggccc ctgattctga gccccgtgct 301 cttccagtac cacactgcct ctgagcctgc cttcgggacc ctactgtgtt ccaggtcccg 361 tctgtccctc agtcacatag ctgtggtcat tccaggacat ggggacaaac cactctttcc 421 cagccaggtg cctttgagcc ttcccttcca agacctgcac ttttcccgct gtgttctctt 481 gtttccagag tccatccatc ttactagctg gggtcgtctt cattatgagc tattccttcc 541 tggagttctc cactgtggtg gagagtgtgg gatgctcaga gggacctgcg tgcatggttt 601 tggtgtgcag agagccatgt cacatcatgt ttctcactgg ggtcttggaa cctgtggatc 661 ccagtcccta tgtgatatgc tcaaatgccc tgtcaacaag ctagaacc //