LOCUS BG088134 758 bp mRNA linear EST 12-MAY-2010 DEFINITION H3149A12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3149A12 5', mRNA sequence. ACCESSION BG088134 VERSION BG088134.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 758) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG088134.1. Other_ESTs: H3149A12-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3149 row: A column: 12 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..758 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3149A12-5" /db_xref="taxon:10090" /clone="H3149A12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 224 a 209 c 192 g 133 t ORIGIN 1 cgcacagcgc aacctccgca gagacaaaga tcggaggaac atggtgcagt tcaacctaca 61 gaccctgccc aagagtgcca agcagaaaga gagagaacga attcgcttgc agaaaaaatt 121 ccagaagcaa tttggagtga ggcaaaaatg ggaccagaag tcacagaaac cccgagactc 181 ctcagttgaa gttcgcagtg actgggaggt gaaggaggag atggacttcc ctcagctgat 241 gaagatgcgc tacttggaag tgtcagagcc tcaagacatc gagtgctgcg gagccctgga 301 gtactacgac aaagcctttg accgcatcac cacaaggagt gagaagcccc tgcggagcat 361 caagcgcatc tttcacaccg tcaccaccac agacgaccct gtcatccgga agctggcaaa 421 aacgcagggc aatgtgtttg ccactgacgc catcctggcc acgctgatga gctgcacccg 481 ctccgtgtac tcctgggaca tcgttgtcca gagagtcggc tctaaactct tctttgacaa 541 gagggacaac tctgactttg acctcctgac tgtgagtgag acagccaatg agccgcctca 601 agatgaaggc aactccttca actcaccccg gaacctggcc atggaagcca cctacatcaa 661 ccacaacttc tctcagcagt gcctgagaat gggaagagaa agatacaatt tccccaaccc 721 aaacccattt gtggaggacg acatggataa gaatgaga //