LOCUS BG088127 605 bp mRNA linear EST 12-MAY-2010 DEFINITION H3149A04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3149A04 5', mRNA sequence. ACCESSION BG088127 VERSION BG088127.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 605) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG088127.1. Other_ESTs: H3149A04-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3149 row: A column: 04 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..605 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3149A04-5" /db_xref="taxon:10090" /clone="H3149A04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 148 a 153 c 171 g 133 t ORIGIN 1 gttaatcagc attggctctt cctataacta tggaaatgaa gaccaggcag aattcctctg 61 tgttgtctcc agagaactaa ataattctac caatggcatc gtcatagagc cgagcgaaaa 121 ggccaagatt cttcaggaga gaggctctcg aatgtaagcg gacagtctga agtttcagaa 181 ccctgaaggc aagagagcaa tcggcccagc agctgcgacg gagatcctgc acctgtacag 241 taaccgagct actgcaaagc aaagctgcgc ctggtcccga gaagtggtgt ccggccggcc 301 aagccagagg agccccaccg cctggatgaa gacagtgcgc ctctggctgc acggtccctt 361 ttcagaaacc tgcttttgtt ttctagccag tgttagaaca gaccttcaca acggcatctg 421 ggatgggtcc ctggctggag cttgtgggga gttgggggga aaggagtaag gtctagctcc 481 ctggaacagt ctagatgctt ttctaaaagc ctgagccgag gaagaaggct tgtcctgagc 541 tgtgcctggt ctcagcctgc gtgcgccatg atggacatga gcagctgctt ggaccattct 601 tccct //