LOCUS BG088110 640 bp mRNA linear EST 12-MAY-2010 DEFINITION H3148G06-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3148G06 5', mRNA sequence. ACCESSION BG088110 VERSION BG088110.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 640) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG088110.1. Other_ESTs: H3148G06-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3148 row: G column: 06 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..640 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3148G06-5" /db_xref="taxon:10090" /clone="H3148G06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 161 a 185 c 160 g 134 t ORIGIN 1 cgagactgtc gtttggtctc ccatccagaa tgtcgggacc gatgacccct tccctgcatc 61 ccccccctgg tggggacacc ggttaagatt ggagagggca tgctggccga cttcgtgtcg 121 caggcttctc ccattatccc tgccattgtc gtcagctgtg tcaatgagat cgagcagcga 181 ggcctgactg aggcaggctt gtacaggatc tcaggctgtg accgcacagt gaaagaactg 241 aaagaaaaat tccttaaggt gaaaactgtg cccctcctca gcaaagtgga cgatatccac 301 gtcatctgca gcctcctgaa ggacttcctg cgcaacctca aagagcccct cctgaccttc 361 tggctgagca aagccttcat ggaggcagca gagataacag atgaagacaa cagcacagcc 421 gccatgtacc aggctgtcag tgagctgccc caggccaaca gggacacgct agccttcctt 481 atgatccacc tacagagagt gtctcagagt ccagacacta agatggatat tgccaatcta 541 gctaaagtct ttggccctac aatagttgcc catactgtgc ccaatccaga tccagtgaca 601 atgttccagg acatcaaacg tcagctcaag gtggtggagc //