LOCUS BG088028 553 bp mRNA linear EST 12-MAY-2010 DEFINITION H3147G04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3147G04 5', mRNA sequence. ACCESSION BG088028 VERSION BG088028.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 553) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG088028.1. Other_ESTs: H3147G04-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3147 row: G column: 04 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..553 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3147G04-5" /db_xref="taxon:10090" /clone="H3147G04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 163 a 122 c 141 g 127 t ORIGIN 1 atttcgcaga gtcgaaggtg aagcgcctga ggaagaagtt tgccctgaag acacttcgaa 61 aggcaaggag gaagctcatc tatgagaagg caaagcacta tcacaaggag tacaggcaga 121 tgtaccgcac tgagattcgg atggccagga tggcaaggaa agctggcaac ttctatgtgc 181 ccgcagaacc aaagctggcc tttgtcatca gaattcgagg tatcaatgga gtaagcccaa 241 aggttcgtaa ggttctgcag ctccttcgtc ttcgacagat cttcaatggc acctttgtta 301 agctcaacaa ggcttcaatt aacatgctgc ggattgtgga gccatacatt gcatgggggt 361 accccaacct gaagtcagta aacgagctca tctacaagcg aggctacggc aaaatcaaca 421 agaagcggat tgccttgaca gataattcct tgattgctcg gtctcttggt aagtttggca 481 tcatctgcat ggaggatcta attcatgaga tctatacagt cgggaaacgc ttcaaggaag 541 caaataactt cct //