LOCUS BG087998 648 bp mRNA linear EST 12-MAY-2010 DEFINITION H3147D05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3147D05 5', mRNA sequence. ACCESSION BG087998 VERSION BG087998.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 648) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG087998.1. Other_ESTs: H3147D05-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3147 row: D column: 05 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..648 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3147D05-5" /db_xref="taxon:10090" /clone="H3147D05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 173 a 152 c 159 g 164 t ORIGIN 1 gtagcctcgg ctatcgagga agcagccctc catcccagtg agctgctgac taacatggaa 61 gatgaggatg acactgatga gactctgact tcactgctca atgaaattgc ttttcttaat 121 caacagctaa atgatgactc tggcctagct gaactgtcag gttctatggg tacagaattc 181 tcaggagatg ctcagcgagc ttttatcagt aaacttgctc ctgggaacag atcagctttc 241 caagttggac acttgggagc aggtgtgaaa gagttgcctg atgtccaaga ggagagtgaa 301 tccatcagcc ccctcctctt gcacctggaa gatgatgact tttctgagaa tgaaaaacag 361 cttggggaca cagcctctga gccagatgtc cttaagattg ttattgaccc tgaaataaag 421 gattctcttg tttcccatag gaaatctagt gatggagggc agagtacttc tggtctcccc 481 gcagagcctg aaagtgtatc ttcacctccc atcttacgca tgaagactgg cccggagaac 541 agcaacacag atactttgtg gaggcctatg ccaaagttgg ccccattagg tttaaaagta 601 gctaatcccc ccagtgatgc cgatggtcag agtctcaagg tgatgcct //