LOCUS BG087745 478 bp mRNA linear EST 12-MAY-2010 DEFINITION H3144D06-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3144D06 5', mRNA sequence. ACCESSION BG087745 VERSION BG087745.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 478) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG087745.1. Other_ESTs: H3144D06-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3144 row: D column: 06 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..478 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3144D06-5" /db_xref="taxon:10090" /clone="H3144D06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 102 a 121 c 115 g 140 t ORIGIN 1 cggcttgggg tctgggattt ccctctttat tgccaccaac atctgtgaaa ccattgtctg 61 gaaggccttt agtcccacta ccattaacac tggcagaggt acggagtttg aaggtgcagt 121 catagctctg tttcatttgc tggccaccag gacagacaag gtgcgagcct tgagggaggc 181 tttctatagg cagaacctcc ccaacctcat gaacctcatc gccacagtgt tcgtgtttgc 241 tgtcgtcatc tattttcagg ggtttcgtgt tgacttgccc attaagtcgg cacggtatcg 301 tggacagtac agtagctatc ctatcaagct cttctacacg tcgaatattc ccataatcct 361 ccagtctgcc ctagtttcaa acttgtacgt catttcccag atgctgtctg ttcgatttag 421 tggcaacttc ttagtaaact tactaggaca gtgggccgat gtcagtgggg gagggcct //