LOCUS BG087703 604 bp mRNA linear EST 12-MAY-2010 DEFINITION H3142C03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3142C03 5', mRNA sequence. ACCESSION BG087703 VERSION BG087703.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 604) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG087703.1. Other_ESTs: H3142C03-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3142 row: C column: 03 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..604 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3142C03-5" /db_xref="taxon:10090" /clone="H3142C03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 112 a 183 c 177 g 132 t ORIGIN 1 ggaagttctg ggctctgttg gaggccccac tccgcttgtg tcctgggatc ggcccagtgt 61 cgggggagat gaggtgcctg gaaccccagc acctctgctg actcgcctcg cccagcacat 121 gaagtggcca tcagtctcat ctctggactc tgccctggaa agcagcccct ccttgcacag 181 ccctgctgac cctggccacc tctctcctcc agcctcctcc cctaggcctt cccggggtca 241 ccgtcgctca gcctcctgtg ggtctccgtt gagtggaaac acaggagaag ggacctctag 301 gagtgctgga tgtgggggcg gggtatctgg gccagggtcc tctgattgcc gaatcatccg 361 agtccagatg gagctggggg aggatggcag cgtctacaag agcatcctgg tgacaagcca 421 ggacaaagct ccaagtgtta ttagtcgtgc ccttaagaaa aacaatcgtg attctgctgt 481 ggcttcagag ttcgagctgg tgcagctgct acctggggat cgagagctga ccatcccaca 541 ctcagctaac gtcttctatg ccatggatgg tgcatctcat gacttcctcc tgcggcagcg 601 caga //