LOCUS BG087677 601 bp mRNA linear EST 12-MAY-2010 DEFINITION H3141G02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3141G02 5', mRNA sequence. ACCESSION BG087677 VERSION BG087677.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 601) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG087677.1. Other_ESTs: H3141G02-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3141 row: G column: 02 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..601 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3141G02-5" /db_xref="taxon:10090" /clone="H3141G02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 115 a 160 c 183 g 143 t ORIGIN 1 atccggccct accttgagag caaggtccac ggtgtacaga agaaggccta ccgcgtgctg 61 gaggaggtgt gtgccagttc tcagggcccg gcagcccgct tcgtgcagag ccacctggat 121 gatctgaaga agactctgct ggactcgctt cggaccacat cctcgcctgc taaaaggccc 181 cgtttgaagt gcctcataca tattgtgaag acgctgtcgg ctgagcatga ggagttcata 241 gctgccctca tcccagaggt gatcctgtgc accaaggagg tgtcagtggg tgcaaggaag 301 agtgcgttta ccttgctggt ggagatgggc catgctttcc tgcgatttgg ctccaaccag 361 gaagacgctc tgcagcggta ccttgtcctc atctatcctg gccttctggg tgcagttacc 421 acagttagtt gcagcatttt ggctctgacc cacctcttgt ttgagttcaa agggctgatg 481 gggaccagca cagtggagca gctcctggag aacgtgtgcc tgctgctggc ctctcgaacc 541 cgtgatgtgg tcaagtccgc gctaggcttc atcaaggttg ctgtggtggt catggacgtg 601 g //