LOCUS BG087580 613 bp mRNA linear EST 12-MAY-2010 DEFINITION H3143C10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3143C10 5', mRNA sequence. ACCESSION BG087580 VERSION BG087580.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 613) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG087580.1. Other_ESTs: H3143C10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3143 row: C column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..613 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3143C10-5" /db_xref="taxon:10090" /clone="H3143C10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 123 a 197 c 150 g 143 t ORIGIN 1 cccctgagaa attccccctg cataccatca atacagtgcg ggtcaacggg caggagaagt 61 acctgattct gtgtgaagtg aatgctgaca gcctgctgga cacctctcta gacactacct 121 gtgatgtcgc ctgcttattg tttgacagca gtgatcccaa gacctttgta cactgtgcta 181 ccatatacaa gcgttattac atggatgggc agaccccctg cctctttatt gcctccaaag 241 ctgatctccc tgaaggtgtt gcccccccag ggctgtcacc cgctgagttc tgccggaggc 301 accggctgcc tgccccagct tcattctcct gcttgggacc cgcaatgccc agcacagatg 361 tctttaccca acttgctacc atggccactt tcccacacct ggtgcacaca gagctgcacc 421 ccacctcctt ctggctccga ggagtgctgg tggctgtcgg cacggctgtg gctgctgtcc 481 ttagcttctc actatacagg gtcctagtga agagccgatg acaaaggacc catgagcctc 541 tcctgccccc aggcacagca cactgtggtg ccttatgtgg gcccacacgg caaagaagta 601 tgggctttcg gcc //