LOCUS BG087389 677 bp mRNA linear EST 12-MAY-2010
DEFINITION H3139A04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3139A04 5', mRNA sequence.
ACCESSION BG087389
VERSION BG087389.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 677)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG087389.1.
Other_ESTs: H3139A04-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3139 row: A column: 04
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..677
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3139A04-5"
/db_xref="taxon:10090"
/clone="H3139A04"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 276 a 128 c 137 g 136 t
ORIGIN
1 gggaagggaa gaagcgtccc ctctccccgc gggccgtggc gactccagaa ggatctgctg
61 acccagcagt gtggccagct tgtcacttag cgccctccaa tccgggctca agagaaaaga
121 aagcaaacca atccagggaa aagaattacc agacaaagaa tgtcaggaga cacagaaagg
181 aagaatgaag tcattcctac aaaagctgca gtcaaagaca catctgcatt aactgatgct
241 gtagagcaag tcgccaaaca acaacagtca caaacatcag aaatagaaaa acataaaaaa
301 attctgttcc agttgcagat tgaacttcat gagcttgaaa aacaaatagc gactattgct
361 gaagaagcta aagaaacaga caggcaaatg catcggcaag atgctgcaat ggagaactct
421 aaacttcagt gtggacgctt agaagctcaa attgagtcct tatattcaga aagtttaaaa
481 cttaaatttg atacagaaac agcccaagaa aaatttgagg agcaaatgat aaaatataat
541 gcatattatg taaaaataaa agcttataaa gataatttag gagagataaa aagccaatgt
601 ccatttatga ctgaactcta tgaaaaacga gatcttatca aaaacctaaa gacaatgaaa
661 gaagatctta tggaaaa
//