LOCUS BG087241 756 bp mRNA linear EST 12-MAY-2010 DEFINITION H3137B11-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3137B11 5', mRNA sequence. ACCESSION BG087241 VERSION BG087241.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 756) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG087241.1. Other_ESTs: H3137B11-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3137 row: B column: 11 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..756 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3137B11-5" /db_xref="taxon:10090" /clone="H3137B11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 186 a 203 c 159 g 208 t ORIGIN 1 caagcagaat cgatctggcc ggctcaccat aaagagtctc cgtctgtcga atggaggtat 61 ctttcctgag acgcatcgtc ctctccttct ccaaaagctc ctcaaagacg gaggtgtgct 121 ttcccccatc ctcaccaggc tcatgaattt cttcgtgttc tctcgaggtc tcactccagt 181 ctttggaccg tatactcgac ccactgagag tgagctgtgg gacatgtggg ctgtgattcg 241 caacaatgac ggcaacctgg tcatcgacag tcttttacag tacattaatc aaaggaagaa 301 tttcagaaga cgctgggtgg gagcgcttgc ttctgtgtcc atccccattc attttatcta 361 tgggccattg gatcctataa atccgtatcc agagtttttg gagctgtaca ggaaaacgct 421 gccgcggtcc acagtgtcga ttctggatga ccacattagc cactacccac agctagagaa 481 tcccatgggc ttcttgaatg catatatggg cttcatcaac tccttctgag ctggaaagag 541 tagcctccct gtattacctc ccccattctc gtatctgtgt gtattccact tagaaatgcc 601 caaaagaggt cctggccatc aaatactatc ctttcatcaa agtccatttc cttcacattg 661 gtgaacaaac tacaggaatc aggcagctgg aactctatag ggtgacacaa tagtccacct 721 catctctaag ggtgacatga taatcctctc atttct //