LOCUS BG087170 617 bp mRNA linear EST 12-MAY-2010
DEFINITION H3136D04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3136D04 5', mRNA sequence.
ACCESSION BG087170
VERSION BG087170.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 617)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG087170.1.
Other_ESTs: H3136D04-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3136 row: D column: 04
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..617
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3136D04-5"
/db_xref="taxon:10090"
/clone="H3136D04"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 154 a 157 c 153 g 153 t
ORIGIN
1 tacccataat gcagtgggta agcggcaacc caagtggtgt gggtgtgtct gctgcttctc
61 tgctctttct gattcgcctt tgtaaatgca ttagggaaaa caatcaaatt ctggagtgag
121 ttttggatgt gatttaaaaa caaatctcca actctgttgc ccacactgtg gggttcccca
181 tcccacacat cttcccttgc agaggcttca ttcacatccc agaagcagag cccagtagct
241 gaacctggct gtcgtgaagg tgaattgatt gcttgtgtgt ctgtctctaa ggtcatcatc
301 tctgtcagcc aactgattgc agatgtggtt ggcattggag gaaccagatt ccagcagtcc
361 ttgtctatca tcaacaactg tgccaacagc gaccggatca tcaagcacac cagcttttcc
421 tctgatgtga aagatttgac taagaggatc cgcacagtcc tgatggccac agcccagatg
481 aaggagcacg agaacgaccc ggagatgctg gtggacctcc agtacagcct ggctaagtcc
541 tacgccagca cccctgagct caggaagacg tggctagaca gtatggcgag gattcacgtt
601 aaaaatgggg acctctc
//