LOCUS BG087170 617 bp mRNA linear EST 12-MAY-2010 DEFINITION H3136D04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3136D04 5', mRNA sequence. ACCESSION BG087170 VERSION BG087170.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 617) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG087170.1. Other_ESTs: H3136D04-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3136 row: D column: 04 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..617 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3136D04-5" /db_xref="taxon:10090" /clone="H3136D04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 154 a 157 c 153 g 153 t ORIGIN 1 tacccataat gcagtgggta agcggcaacc caagtggtgt gggtgtgtct gctgcttctc 61 tgctctttct gattcgcctt tgtaaatgca ttagggaaaa caatcaaatt ctggagtgag 121 ttttggatgt gatttaaaaa caaatctcca actctgttgc ccacactgtg gggttcccca 181 tcccacacat cttcccttgc agaggcttca ttcacatccc agaagcagag cccagtagct 241 gaacctggct gtcgtgaagg tgaattgatt gcttgtgtgt ctgtctctaa ggtcatcatc 301 tctgtcagcc aactgattgc agatgtggtt ggcattggag gaaccagatt ccagcagtcc 361 ttgtctatca tcaacaactg tgccaacagc gaccggatca tcaagcacac cagcttttcc 421 tctgatgtga aagatttgac taagaggatc cgcacagtcc tgatggccac agcccagatg 481 aaggagcacg agaacgaccc ggagatgctg gtggacctcc agtacagcct ggctaagtcc 541 tacgccagca cccctgagct caggaagacg tggctagaca gtatggcgag gattcacgtt 601 aaaaatgggg acctctc //