LOCUS BG086850 576 bp mRNA linear EST 12-MAY-2010 DEFINITION H3131C10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3131C10 5', mRNA sequence. ACCESSION BG086850 VERSION BG086850.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 576) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG086850.1. Other_ESTs: H3131C10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3131 row: C column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..576 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3131C10-5" /db_xref="taxon:10090" /clone="H3131C10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 127 a 161 c 145 g 143 t ORIGIN 1 acctgaacgt gcgggacacg cactcgctgt tcttccgcag cctgaaggac cggggctttg 61 agctcacctt caagaccgca gatgacccca gcttgtccct cattaagtac ggggagttcc 121 tctatgacaa ccttatcatc ttttccccgt cggtggaaga ttttggaggc aacatcaacg 181 tggagaccat cagtgccttc attgatggtg gcggcagcgt tttggtggct gccagctctg 241 atattggtga ccctcttcgg gagctgggca gtgagtgtgg gattgaattt gatgaagaga 301 aaacagctgt catcgaccac cacaactatg atgtttctga ccttggccag cacacactca 361 ttgtggctga cactgagaac ctgctgaagg ccccgaccat tgttggcaag tcatctctga 421 accccattct cttccgagga gttggaacgg tggcagaccc ggacaatccc ttggttttgg 481 acatcctaac aggctcttca acctcttact ccttcttccc agataaacca atcacccagt 541 acccccatgc ggtggggagg aacactctgc tgattg //