LOCUS BG086780 621 bp mRNA linear EST 12-MAY-2010 DEFINITION H3130D12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3130D12 5', mRNA sequence. ACCESSION BG086780 VERSION BG086780.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 621) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG086780.1. Other_ESTs: H3130D12-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3130 row: D column: 12 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..621 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3130D12-5" /db_xref="taxon:10090" /clone="H3130D12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 149 a 120 c 123 g 229 t ORIGIN 1 ctttctctct gtacacaaag tatccaaata ttatacctta aggattccta ccaggttttc 61 ttgaaaagtg gcttttacat gaggcaagaa ggaagtagtt gtagatagaa tcattttcta 121 gttctttaaa tgggacaatc actttgtttt ctaatagatg tgacctgtgt aacttaaaaa 181 atctgagggt ttgagagagg ttgctctaga tttccaatga cgataccctg gctgaatagg 241 cttttctctt ccttgggcct ttacttcttt tcttcctcct tggtttcagt tcccatgtgc 301 tatcattata atcatgcaga cttttacttc agttgcttct gaaagcacta agttttcagt 361 tttggagtta cttctccctt gcctctgaca cactgcacac gtctagaggc tgtaattgga 421 aataggcttt tggtttcctg tgccattcaa gtttggggct ttcatttggt tacagagttc 481 agcgcttggt tcccagggtt tatatacgaa acagcggatt gctgtgggct ggttcataaa 541 gtgaaactaa tgaatctatc tctttgtcac ttgctggtac agctagtttg tttcacttgc 601 acatttgtac atatatctaa t //