LOCUS BG086708 636 bp mRNA linear EST 12-MAY-2010 DEFINITION H3129F04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3129F04 5', mRNA sequence. ACCESSION BG086708 VERSION BG086708.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 636) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG086708.1. Other_ESTs: H3129F04-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3129 row: F column: 04 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..636 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3129F04-5" /db_xref="taxon:10090" /clone="H3129F04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 197 a 131 c 152 g 156 t ORIGIN 1 caggacagcc tcgccttgcc cctctaccac ctcttcctga atatggagga aaagtgcgtc 61 ttgggctgat tcctgaggaa tttttccagt tcctttaccc taagactggt gtaacaggac 121 cttatgtgct tggaactgga cttagcttgt attttctatc caaagaaata tatgtgatta 181 ccccagagac cttctctacc atatcagtag tagggttgat agtctatgtg attaagaaat 241 atggcgcctc ttttggagaa tttattgaca aacttaatga ggaaaaaatt gctcaactag 301 aagaagtaaa gcagtcgagc atgaaacaaa tccaggatgc aatcgacatg gagaaggcac 361 agcaggcact ggttcagaag cgccattacc tcttcgatgt gcagaggaat aacattgccc 421 tggccttgga ggtcacttac cgggaacggc tacataaagc atataaggag gtaaagaatc 481 gcctggacta ccacatctct gtacagaaca tgatgcgtcg caaggaggaa gaacacatga 541 tagactgggt agaaaagcat gtggtgaaga gcatttctgt acagcaggaa aaggagacca 601 ttgccaagtg cattgaagat ctaaagctgc ttgcaa //