LOCUS BG086517 719 bp mRNA linear EST 12-MAY-2010 DEFINITION H3127C01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3127C01 5', mRNA sequence. ACCESSION BG086517 VERSION BG086517.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 719) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG086517.1. Other_ESTs: H3127C01-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3127 row: C column: 01 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..719 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3127C01-5" /db_xref="taxon:10090" /clone="H3127C01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 236 a 169 c 154 g 160 t ORIGIN 1 ggctcgtggg cctccagaat gtgttttccc accagctgcc cagaatgccc aaagagtaca 61 tcacacggct cgtctttgac ccgaaacaca aaacccttgc tttaattaaa gatggccgtg 121 tcattggtgg tatctgtttc cggatgtttc catcccaggg attcacagag attgttttct 181 gtgcagtaac ctcaaatgaa caagtcaagg gctatggaac ccacctgatg aaccatctca 241 aagaatacca cataaagcac gagatcctca acttcctcac atatgcagat gagtatgcca 301 tcggctattt caagaagcag ggtttctcca aagaaatcaa aatacctaaa accaaatatg 361 ttggctacat caaggattat gaaggggcca ctttgacggg atgtgagctg aaccctcaga 421 tcccatacac agagttctct gtcatcatta aaaagcagaa ggagatcatt aaaaagctga 481 tagaaagaaa acaagcccag attcgaaaag tctaccctgg actttcgtgt ttcaaagatg 541 gagttcggca gattcctata gaaagcattc ctggaatcag agagacaggc tggaaaccaa 601 gtggaaaaga gaaaagtaaa gagcccaaag accctgagca gctttacagc accctcaaga 661 acatcctgca gcaggtgaag aaccatccaa atgcttggcc tttcatggaa ccagtgaag //