LOCUS BG086504 604 bp mRNA linear EST 12-MAY-2010 DEFINITION H3127A08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3127A08 5', mRNA sequence. ACCESSION BG086504 VERSION BG086504.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 604) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG086504.1. Other_ESTs: H3127A08-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3127 row: A column: 08 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..604 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3127A08-5" /db_xref="taxon:10090" /clone="H3127A08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 176 a 167 c 147 g 114 t ORIGIN 1 gggggatttt ctgaacttca gtcgcgcctt attcgttacg agacgcagac cacctgcacc 61 agagacagtt ttccagtccc caccgtgctg agccctcttc catcccctgc agttctgtca 121 gagcctcaga gtgtccccga aggagaagca ctgcaaggcg aactgaggac tgaagtttct 181 ggattgaagc ggagatctaa agaccccagc tgcctgtacc cccagaaaag acttacgaga 241 tcagaaagtt ctgattgttt gccttcccaa gcgagttgca atagtaatca tcaccatcac 301 acagggaaac ccaggaagcc tcaggcagag cgctgtgtgt cagggcttcc tctgcctggc 361 cgggaagctt ccaaagatac ctcaaagacc agttcaggac aaaaacgagc acacgaatca 421 aaatcatcaa agcaaatgaa ggaatcacgg tcccagaaac acacacggat gctgaaggag 481 gtggtaaaag acaccttgaa gaggcaccac atcacggagg cccatgagag cttcacggcc 541 tgcagccaga gactcttcga catctccaag ttctacctga aggatctgaa aacatcaagg 601 ggtc //