LOCUS       BG086501                 710 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3127A05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3127A05 5', mRNA sequence.
ACCESSION   BG086501
VERSION     BG086501.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG086501.1.
            Other_ESTs: H3127A05-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3127  row: A  column: 05
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3127A05-5"
                     /db_xref="taxon:10090"
                     /clone="H3127A05"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          148 a          193 c          216 g          153 t
ORIGIN      
        1 atggccacgt acttaaccct gagcagcctg gagagccaca gcaggaagtg catatgctat
       61 ggatgcctgt ccccccagcc ttgacccttg gggaagagga gaaggacagg acctgggagt
      121 ttctgactgt tgtgggtagc agccaggctg aagcccagga ctgctttgct gaggccctac
      181 agctgcagac caggggtgtt ctgtatacaa tccatgcaga ctcctggggc cggctctggg
      241 caggctgtgg cctggatgtg gctgggcccc tggccctgcg ccaggccctt cgtggctctc
      301 tctactacct gttcagtgag ctcccccagc ctggcactca aggattcatc agccatgggc
      361 ttagccccgg aggactctcc aatgggagta aggaggaatg ctactggggc catatcttct
      421 gggatcagga catctggatg ttcccaaata ttttgatgtt ccacccagaa gccgccaggg
      481 ccatcctaga ataccgagtc cgtacgctgg gaggagccct gaagaatggc cagaacctgg
      541 gctaccaggg agccaagttt gcttgggaga gtgcaagcac tggcctggag gtctgccctg
      601 aggacattta tgggacccag gagatccata tcaatggggc tgtggccttg gcctttcagc
      661 tgtattacta ttacactcag gactcgaagc tcttccaaga ggatggtggc
//