LOCUS BG086499 647 bp mRNA linear EST 26-JAN-2001 DEFINITION H3127A03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3127A03 5', mRNA sequence. ACCESSION BG086499 VERSION BG086499.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 647) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3127A03-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3127 row: A column: 03 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..647 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3127A03-5" /db_xref="taxon:10090" /clone="H3127A03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 182 a 161 c 142 g 162 t ORIGIN 1 aaaaacatca ttatatactt ctgacatacc atggcagagt atatattcct cttcccaaag 61 ggagacatag ggagatccta aggaaatact aaatcaagat taaaacgcag caaggcacac 121 agtaagtcct gttgctaggt gcacaatatc tagggcttca ctttgtaagg gtttaggtgg 181 cagtgcccct ccagctttgg tgcctgcaac atgcatctct tttgagctgt ttcgggttcc 241 tctgttcagc cctcctaggg agacttctca tggctgtggt atcttcaaca tcatagagta 301 tccatcacaa tccagacttt tatttccata gcttaattca gtgacctttc aaggcctgct 361 tgcaaggaag tagacccagc catgtgctac ctacctctgc agctctctaa aggaagaact 421 catggtcccc cgccccaatc tcacattttt attgtcttta aaatatggcc tcttagccgg 481 gcgtggtggc gcatgccttt aatcctagca ctcgggaggc agaggcaggc ggatttctga 541 gtttgaggcc agcctggtct acagagtgag cccaggacag ccagggctac acagaaaaac 601 cctgtctcga aaaaccaaaa aaaaaaaaaa aaaaaaaggg cggccgc //