LOCUS BG086229 573 bp mRNA linear EST 12-MAY-2010 DEFINITION H3123C11-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3123C11 5', mRNA sequence. ACCESSION BG086229 VERSION BG086229.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 573) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG086229.1. Other_ESTs: H3123C11-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3123 row: C column: 11 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..573 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3123C11-5" /db_xref="taxon:10090" /clone="H3123C11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 140 a 182 c 171 g 80 t ORIGIN 1 gtcccggctg cccaggacac cactgacaag aagcccacca ggccagcagc agcatcgcag 61 gactcacaag cggctgacgg agcaggcaga gcctctttgg ccacgaagag gaaagaggac 121 cccgcagacc ctgaagtcaa ccagaggaac ctccagtttg cgggagtcaa tggcgggtat 181 gagtccccct cggaggactc cagcacagca gaaaactccg aacacgagag cacagagaac 241 gagggcccgg agccaccagc aagggttctg agcccagctg aatgtcctca gctcaggcct 301 ccaggggctg cagtggccac gaccagtctg gaggggcccc agctctccca ggaatcccag 361 cgtgtgcctg cacctgaggt ggcgtcagga cccgatcctg aggaggaaat tcggatggat 421 ctgagccctg acctcatatc agcctgcttg gccctggaga aatacctgga gaaccccaat 481 gccctcacag agcgagaact gaaagtggcc tataccacag tgcttcagga gtggctgcgc 541 ctggcctgcc gcagtgacgc acaccctgag ctt //