LOCUS BG086218 538 bp mRNA linear EST 12-MAY-2010
DEFINITION H3123B09-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3123B09 5', mRNA sequence.
ACCESSION BG086218
VERSION BG086218.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 538)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG086218.1.
Other_ESTs: H3123B09-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3123 row: B column: 09
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..538
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3123B09-5"
/db_xref="taxon:10090"
/clone="H3123B09"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 136 a 132 c 137 g 133 t
ORIGIN
1 aagtgaactc aatgcataca gtcatgaagt cattaccatc cttggagaga ttgattttat
61 gttggggaat cttcctgaat tggcttctgc gagaccggct aagaagaacc tgctaaccat
121 gatggatgag gcctacgttc agcccgagcc tctgggagtc gtactgatta ttggagcttg
181 gaattaccct ttcgttctta ccatgcaacc gctggtggga gccattgctg caggaaatgc
241 tgccattgtt aagccctcag aactcagtga aaacacggcc aagatcttgg ctgaactcct
301 ccctcagtac ttagaccagg acctgtatgc gattgttaat ggcggtatcc cggaaaccac
361 ggagcttctg aagcagcggt ttgaccacat tctctataca gggaacactg cagttggaaa
421 aattgtcatg gaagctgctg ccaagcacct gacccctgtg accctggaac tcggcgggaa
481 aagcccttgt tacattgaca gagactgtga tctggacgtg gcttgcagac gcatagcc
//