LOCUS BG086125 592 bp mRNA linear EST 12-MAY-2010 DEFINITION H3122A12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3122A12 5', mRNA sequence. ACCESSION BG086125 VERSION BG086125.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 592) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG086125.1. Other_ESTs: H3122A12-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3122 row: A column: 12 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..592 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3122A12-5" /db_xref="taxon:10090" /clone="H3122A12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 123 a 152 c 183 g 134 t ORIGIN 1 gctccagcag ggaccccgag catcatgagg agggcagctg aagctgggtg tccctggagt 61 gggactttgg acaacgaagg agaatttgga aatggattgt ctttaatagc tggagcctcc 121 caaggccact atgtgtcccc tactggttga agatcctgca agtgtcccct actggcttca 181 tggggctggt gatggcagat tttgccatga accaagcttt gcatgttgaa gctgatgctg 241 tgttccagcg ggcctgaccc tgggagtcgc tctgctgctt tctgagggtc tccctggagc 301 cgtggtcatt cccaggcacc gcacacccaa ggccagagct tggccagctt taacaggagt 361 aatgctcgcc gcaccacctc ctgccgaggg gcatgagtag gagcagctgt cacagggaga 421 aaggaggcag attgtccctt catctacagc cctggaagga aggctggtgc ccttgaaatg 481 tgaagaactt tgccctctgc ctctgctgtg gatatgggcc accttacttt gacgacctgc 541 acaccaagca agccttggtc atttcaggaa gggtgtgggg caggatgggg gt //