LOCUS BG086101 771 bp mRNA linear EST 12-MAY-2010
DEFINITION H3121G07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3121G07 5', mRNA sequence.
ACCESSION BG086101
VERSION BG086101.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 771)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG086101.1.
Other_ESTs: H3121G07-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3121 row: G column: 07
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..771
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3121G07-5"
/db_xref="taxon:10090"
/clone="H3121G07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 257 a 133 c 212 g 169 t
ORIGIN
1 aacaaagttg gcaggcgaag agcctcttgt gaattttcat gtgggtctta aagaagagaa
61 ttgtactact ggtgattcag ttaagtctga ggctgagttg caagaagctt cccttccacc
121 tgaaatagta actgtgaaag agaagactta cgatacagac gctagtgaag cagtgtctga
181 aatccaaggg ccatgtagtg agaaccacag ccctgctgag gacccaggct taagtgagtg
241 caaagacatt tcacagaagc agctttcaga gaacggagag cttgacatca gcgatgtagg
301 gaaggcatgc aaagtcatag ctgggtccag tccagaggga gttgaaacta tggaattaaa
361 tgtaagaaat gatgcatttg tagctgctga ctctgaaaag agtacccaaa tggatgtttc
421 tgtagatgtg gcaacagagg aagataataa aaaagatgaa tgtgaagcag tcacaactga
481 agtgaatgtt gaaggagtag ccactgagga ctttaattca ggtatggatc tttctgatac
541 tcccatacct gtaagcaagg atgttgagac tgagcacgca gctagtgggg aaatagaggg
601 ggagagcaat gaatctgatt caggctcatg tgaggaaatg aacaaagaaa tgggaagtca
661 caaagcccag atgagcactg aaattgacag tgccagggta aaggagacgg acatacttgc
721 cagtgcctct aaatctgaag aagcgctgat tggaagacta gacgtcacac c
//