LOCUS BG086079 713 bp mRNA linear EST 12-MAY-2010
DEFINITION H3121E08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3121E08 5', mRNA sequence.
ACCESSION BG086079
VERSION BG086079.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 713)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG086079.1.
Other_ESTs: H3121E08-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3121 row: E column: 08
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..713
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3121E08-5"
/db_xref="taxon:10090"
/clone="H3121E08"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 182 a 200 c 198 g 133 t
ORIGIN
1 accgcctaaa gaagacgtgg gaagaggtct ccagggacag ctttcgagtg ttccagaaac
61 tgtcggagat cttctctgat gagaacaact actccctgag cagagagctg ctcatcaagg
121 aaggaacctc caagtttgcc acactggaga tgaaccctag gagagcccag aggcggcaga
181 aggagacagg agtcatccag ggcaccgttc cctacctggg cacattcctc actgacctgg
241 tgatgctgga cactgccatg aaggactatc tctatgggag actgatcaac tttgaaaaga
301 gaaggaagga gttcgaagtc attgcccaga tcaagttgct acagtcggcc tgcaacaact
361 acagcattgc tccggaagaa cactttggaa catggttccg agctatggag cgactcagtg
421 aggctgagag ctacaccctg tcgtgtgagc tggagccccc gtctgagtcg gccagcaaca
481 ccctgaggag caagaaaagc acagccattg tcaagcgctg gagcgaccgc caggctccca
541 gcacggagct cagcaccagt agcagtgccc actccaagtc ctgtgaccag cttcggtgca
601 gcccttacct cggcagcggg gacatcaccg acgcgctcag tgtgcactca gctggctctt
661 ccagctctga tgtggaggag atcaacatga gcttcgtccc agagtctcct gat
//