LOCUS BG086069 485 bp mRNA linear EST 12-MAY-2010 DEFINITION H3121D09-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3121D09 5', mRNA sequence. ACCESSION BG086069 VERSION BG086069.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 485) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG086069.1. Other_ESTs: H3121D09-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3121 row: D column: 09 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..485 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3121D09-5" /db_xref="taxon:10090" /clone="H3121D09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 127 a 135 c 119 g 104 t ORIGIN 1 ggaagccgca gggagaagct gttctcaccg ctactctgag catcttcctg ctgcgtggag 61 ctaccgctga caccatgttg aagaaattcg acaagaagga cgaggagtct ggctcgggtc 121 tttaacgaaa ctcccatcaa tccccggaaa tgtgctcaca tcctcaccaa gatcctttat 181 ctcataaacc agggggagca cctggggacc acggaagcaa ctgaggcttt ctttgccatg 241 accaagctct tccagtccaa tgatcccaca ctccgccgca tgtgctattt gaccatcaag 301 gagatgtcct gcatcgctga ggatgtcatc attgtgacaa gcagcctgac aaaagacatg 361 actgggaaag aggataatta ccgcggtcct gctgtccgtg ccctctgcca gatcactgac 421 agcaccatgc tgcaggctga tgaacgctac atgaaacaag caattgtgga caaggttccc 481 agtgt //