LOCUS BG086035 689 bp mRNA linear EST 12-MAY-2010 DEFINITION H3121A02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3121A02 5', mRNA sequence. ACCESSION BG086035 VERSION BG086035.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 689) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG086035.1. Other_ESTs: H3121A02-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3121 row: A column: 02 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..689 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3121A02-5" /db_xref="taxon:10090" /clone="H3121A02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 121 a 216 c 196 g 156 t ORIGIN 1 gcggctggtt tgtcagcaac ctggagttct acgacctgca ggacagcctt tccgacggca 61 ccctcatcgc catggggctc tcggtggccg ttgccttcag cgtgatgctg ctgaccacgt 121 ggaatatcat cataagcctg tacgtcatcg tctccatagc tggaaccata tttgtcacag 181 tcgggtccct ggtcctgctg ggctgggagc tgaacgtgct ggagtccgtc accatctcgg 241 tcgcagtcgg cctgtctgta gactttgccg tccactacgg ggtggcgtac cgcttggctc 301 cagatcccga ccgagaaggc aaggtcatct tctctctgag ccgtatgggc tctgcgatcg 361 ccatggctgc actgaccacc ttcgtggcgg gggccatgat gatgccctcc acggtcctgg 421 cttacacgca gctgggcacg ttcatgatgc tcgtcacgtg cgtgagctgg gccttcgcca 481 ccttcttctt ccagtgcctg tgtcggtgcc tggggccaca aggcacctgc ggccagatcc 541 ctttccctac aaaactccag tgcagtccct tttcccacac cttgtctgca aggcctgggg 601 acaggggacc aagcaaaaca catgccgcca gtgcgtacag cgtagatgcc aggggccaga 661 aatcccaact ggagcatgag ttttacgag //