LOCUS BG086024 624 bp mRNA linear EST 12-MAY-2010 DEFINITION H3120H03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3120H03 5', mRNA sequence. ACCESSION BG086024 VERSION BG086024.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 624) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG086024.1. Other_ESTs: H3120H03-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3120 row: H column: 03 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..624 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3120H03-5" /db_xref="taxon:10090" /clone="H3120H03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 196 a 148 c 141 g 139 t ORIGIN 1 gccagaaaac aatttattcc gaacaagaaa tctttgatat ttactatcct acagagcagg 61 tgacaataca agtgctgcca ccaaaaaatg ccatcaaaga aggggacaac atcacccttc 121 agtgtttggg gaatggcaac ccacctcctg aggagttcat gttttactta ccaggacagc 181 ctgaaggcat aagaagctca aacacttaca cactgacaga tgtgagacgc aatgccacag 241 gggactacaa atgctcactc attgacaaaa ggaacatggc ggcttcaacg accatcacag 301 ttcactactt ggatttatcc ttaaacccaa gtggggaggt gaccaagcag attggcgata 361 ccctgcctgt gtcatgcaca atatctgcaa gtaggaacgc gactgtggtg tggatgaagg 421 ataacatccg gctccggtcc agcccttcct tttccagtct tcattatcag gatgctggga 481 actatgtctg tgaaactgct cttcaggagg ttgagggact gaagaaaagg gagtcgctga 541 ccctcattgt agaagggaaa cctcaaatca aaatgactaa gaaaactgac cccagtggac 601 tgtctaaaac tataatctgc cacg //