LOCUS BG085935 597 bp mRNA linear EST 12-MAY-2010
DEFINITION H3119H03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3119H03 5', mRNA sequence.
ACCESSION BG085935
VERSION BG085935.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 597)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG085935.1.
Other_ESTs: H3119H03-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3119 row: H column: 03
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..597
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3119H03-5"
/db_xref="taxon:10090"
/clone="H3119H03"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 70 a 190 c 197 g 140 t
ORIGIN
1 ggtgatgctg gtcccaaagg tgctgatggt tctcctggta aagatggtgc ccgtggtctg
61 actggtccca ttggtcctcc tggccctgct ggtgcccccg gtgacaaggg tgaagctggt
121 cccagtggtc ctccctttcc caccggagcc cgtggtgctc ccggagaccg tggtgaggct
181 ggtccccctg gtcctgctgg ctttgccggc ccccctggtg ctgatggcca acctggtgcg
241 aaaggtgaac ctggtgatac tggtgttaaa ggtgatgctg gtcctcctgg ccctgctggt
301 cctgctggac cccccggccc cattggtaac gttggtgctc ctggacccaa aggtcctcgt
361 ggtgctgctg gtccccctgg tgctactggc ttccctggtg ctgctggccg tgtcggtccc
421 cctggtccct ctggaaatgc tggaccccct ggccctcccg gtcccgttgg caaagaaggg
481 ggcaaaggtc cccgtggtga gactggccct gctggacgtc ctggtgaagt tggtccccca
541 ggtccccccg gtcctgctgg tgagaaagga tctcctggtg ctgatggacc tgctggc
//